R&D Systems™ Recombinant Human CD27/TNFRSF7 Fc Chimera Protein

Catalog No.	Quantity
382CD100	100 μg

Catalog No. 382CD100 Supplier R&D Systems™ Supplier No. 382CD100

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity

Measured by its ability to inhibit the proliferation of mouse T cells induced by CD27 Ligand and anti-CD3. The ED50 for this effect is 0.75-3 μg/mL in the presence of 10 μg/mL (100 μL/well) Recombinant Mouse CD27 Ligand/TNFSF7 (Catalog # 783-CL ) and anti-CD3.

Specifications

• Accession Number: P26842
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS.
• Gene ID (Entrez): 939
• Molecular Weight (g/mol): M.W. (Observed): 60-70 kDa, reducing conditions; M.W. (theoretical): 46.5 kDa (monomer)
• Name: CD27/TNFRSF7 Fc Chimera
• Quantity: 100 μg
• Source: Mouse myeloma cell line, NS0-derived human CD27/TNFRSF7 protein Human CD27 (Thr21-Ile192) (N-terminus) Accession # P26842 EGRMD Human IgG1 (Pro100-Lys330) 6-His tag (C-terminus)
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Structural Form: Disulfide-linked homodimer
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Gene Symbol: CD27
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Reconstitution: Reconstitute at 100 μg/mL in sterile PBS.
• Purity or Quality Grade: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Protein: CD27/TNFRSF7