R&D Systems™ Recombinant Human CD277/BTN3A1 Fc Chimera Protein

Description

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Measured by its ability to inhibit anti-CD3 antibody induced IL-2 secretion in human T lymphocytes. The ED50 for this effect is typically 1-5 μg/mL.

Specifications

• Accession Number: O00481
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS.
• Gene ID (Entrez): 11119
• Molecular Weight (g/mol): M.W. (Observed): 54-62 kDa, reducing conditions; M.W. (theoretical): 51 kDa
• Quantity: 50 μg
• Source: Human embryonic kidney cell, HEK293-derived human CD277/BTN3A1 protein Human CD277/BTN3A1 (Phe31-Gly254) (N-terminus) Accession # O00481 IEGRMD Human IgG1 (Pro100-Lys330) (C-terminus)
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Gene Alias: BT3.1, BTF5, BTN3A1, butyrophilin, subfamily 3, member A1, CD277, dJ45P21.3 (butyrophilin, subfamily 3, member A1)
• Common Name: CD277/BTN3A1
• Gene Symbol: BTN3A1
• Biological Activity: Measured by its ability to inhibit anti-CD3 antibody induced IL-2 secretion in human T lymphocytes. The ED50 for this effect is typically 1-5μg/mL.
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Reconstitution: Reconstitute at 100 μg/mL in PBS.
• Expression System: Human embryonic kidney cell, HEK293-derived Human CD277/BTN3A1 (Phe31-Gly254) Accession # O00481, N-terminus; IEGRMD; Human IgG1 (Pro100-Lys330), C-terminus
• Purity or Quality Grade: >90%, by SDS-PAGE with silver staining.
• Protein: CD277/BTN3A1