R&D Systems™ Recombinant Human CD3 delta/CD3d Fc Avi-tag Protein

Description

Biotinylated

The biotin to protein ratio is greater than 0.7 as determined by the HABA assay. Measured by its binding ability in a functional ELISA. When Recombinant Human CD3 epsilon Fc Chimera Protein (Catalog # 9850-CD ) is immobilized at 10 μg/mL, 100 μL/well, the concentration of Biotinylated Recombinant Human CD3 delta/CD3d Fc Chimera Avi-tag (Catalog # AVI10280) that produces 50% of the optimal binding response is approximately 2.0-14 μg/mL.

Specifications

• Accession Number: P04234.1
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Gene ID (Entrez): 915
• Molecular Weight (g/mol): M.W.-observed: 54-61 kDa, under reducing conditions, M.W.-theroretical: 38 kDa
• Quantity: 50 μg
• Source: Chinese Hamster Ovary cell line, CHO-derived human CD3 delta/CD3d protein Human CD3 delta/CD3d (Phe22-Ala105) (c-terminus) Accession # P04234.1 IEGRMD Human IgG1 (Pro100-Lys330) Avi-tag N-terminus C-terminμs
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Gene Alias: CD3 antigen, delta subunit, CD3D, CD3d antigen, CD3d antigen, delta polypeptide (TiT3 complex), CD3d molecule, delta (CD3-TCR complex), CD3-DELTA, T3DOKT3, delta chain, T-cell receptor T3 delta chain, T-cell surface glycoprotein CD3 delta chain
• Conjugate: Biotin
• Reconstitution: Reconstitute at 500 μg/mL in PBS.
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: CD3 delta