R&D Systems™ Recombinant Human CD3 gamma Fc Chimera Protein

Catalog No.	Quantity
10550CD050	50 μg

Catalog No. 10550CD050 Supplier R&D Systems™ Supplier No. 10550CD050

Description

Carrier Free Recombinant Protein

Measured by its binding ability in a functional ELISA. When Recombinant Human CD3 gamma Fc Chimera (Catalog # 10550-CD) is immobilized at 1.0 μg/mL, 100 μL/well, the concentration of Biotinylated Recombinant Human CD3E Fc Chimera that produces 50% of the optimal binding response is approximately 0.5-4 μg/mL.

Specifications

• Accession Number: P09693.1
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Gene ID (Entrez): 917
• Molecular Weight (g/mol): M.W.-observed: 42-53 kDa, under reducing conditions, M.W.-theoretical: 37 kDa
• Quantity: 50 μg
• Source: Human embryonic kidney cell, HEK293-derived human CD3 gamma protein Human CD3 gamma (Gln23-Ser116) (N-terminus) Accession # P09693.1 IEGRMD Human IgG1 (Pro100-Lys330) (C-terminus)
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Gene Alias: CD3g, CD3g antigen, CD3g antigen, gamma polypeptide (TiT3 complex), CD3g molecule, epsilon (CD3-TCR complex), CD3g molecule, gamma (CD3-TCR complex), CD3-GAMMA, FLJ17620, FLJ17664, FLJ79544, FLJ94613, IMD17, MGC138597, T3G, T-cell antigen receptor complex, gamma subunit of T3, T-cell receptor T3 gamma chain, T-cell surface glycoprotein CD3 gamma chain
• Conjugate: Unconjugated
• Reconstitution: Reconstitute at 250 μg/mL in PBS.
• Purity or Quality Grade: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: CD3 gamma