R&D Systems™ Recombinant Human CD30/TNFRSF8 Fc Alexa Fluor™ 647 Protein

Description

Fc Chimera

Measured by flow cytometry for its ability to bind HEK293 human embryonic kidney cells transfected with human CD30 Ligand. The concentration of Recombinant Human CD30/TNFRSF8 Fc Chimera Alexa Fluor™ 647 (Catalog # AFR11155) that produces 50% of the binding response is 0.500-5.00 ng/mL.

Specifications

• Accession Number: P28908.1
• For Use With (Application): Bioactivity
• Formulation: Supplied as a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Gene ID (Entrez): 943
• Molecular Weight (g/mol): M.W.-observed: 80 - 110 kDa, under reducing conditions., M.W.-theroretical: 65 kDa
• Quantity: 20 μg
• Source: Human embryonic kidney cell, HEK293-derived human CD30/TNFRSF8 protein Human CD30 (Phe19-Lys379) (C-terminus) Accession # P28908.1 IEGRMD Human IgG1 (Pro100-Lys330) (N-terminus)
• Storage Requirements: Protect from light. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after opening. 3 months, -20°C to -70°C under sterile conditions after opening.
• Endotoxin Concentration: <1.0 EU / 1 μg of the protein by the LAL method.
• Gene Alias: CD30, CD30 antigen, CD30KI-1, CD30L receptor, cytokine receptor CD30, D1S166EKi-1, Ki-1 antigen, Lymphocyte activation antigen CD30, TNFRSF8, tumor necrosis factor receptor superfamily member 8, tumor necrosis factor receptor superfamily, member 8
• Conjugate: Alexa Fluor 647
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: CD30/TNFRSF8