R&D Systems™ Recombinant Human CD40 Ligand/TNFSF5 (HEK293-expressed)

Catalog No.	Quantity
6420CLB050	50 μg

Catalog No. 6420CLB050 Supplier R&D Systems™ Supplier No. 6420CLB050

Description

HA tag

Measured in a cell proliferation assay using human B cells. The ED50 for this effect is 0.25-300 ng/mL in the presence of Recombinant Human IL-4 (Catalog # 204-IL ) and a cross linking HA Tag Antibody (Catalog # MAB060 ).

Specifications

• Accession Number: P29965.1
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS and EDTA with Trehalose.
• Gene ID (Entrez): 959
• Molecular Weight (g/mol): M.W.-observed: 20-27 kDa, under reducing conditions., M.W.-theroretical: 22 kDa
• Quantity: 50 μg
• Source: Human embryonic kidney cell, HEK293-derived human CD40 Ligand/TNFSF5 protein YPYDVPDYA GCN4-IZ GGGSGGGSGGGS Human CD40 Ligand (Met113-Leu261) (C-terminus) Accession # P29965.1 (N-terminus)
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Gene Alias: CD154, CD154 antigen, CD40 antigen ligand, CD40 ligand, CD40L, CD40-L, CD40LG, CD40LIGM, gp39, hCD40L, HIGM1, T-B cell-activating molecule, T-BAM, T-cell antigen Gp39, TNF-related activation protein, TNFSF5, TNFSF5IMD3, TRAP, TRAPtumor necrosis factor (ligand) superfamily, member 5 (hyper-IgM syndrome), tumor necrosis factor (ligand) superfamily member 5, Tumor necrosis factor ligand superfamily member 5
• Conjugate: Unconjugated
• Reconstitution: Reconstitute at 500 μg/mL in PBS.
• Purity or Quality Grade: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Protein: CD40 Ligand/TNFSF5