R&D Systems™ Recombinant Human CD47 Fc Chimera Protein

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity

Measured by its binding ability in a functional ELISA. When Recombinant Human (rh) SIRP alpha/CD172a Fc Chimera (Catalog # 4546-SA ) is coated at 2 μg/mL, Recombinant Human CD47 Fc Chimera binds with an apparent Kd <1 nM.

Optimal dilutions should be determined by each laboratory for each application.

Also measured by its ability to antagonize red blood cell adhesion to immobilized rhSIRP alpha Fc Chimera. The ED50 for this effect is 0.5-2.0 μg/mL.

Specifications

• Accession Number: Q08722
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS.
• Gene ID (Entrez): 961
• Molecular Weight (g/mol): M.W. (Observed): 60-65 kDa, reducing conditions; M.W. (theoretical): 40.3 kDa (monomer)
• Name: CD47 Fc Chimera
• Quantity: 50 μg
• Source: Mouse myeloma cell line, NS0-derived human CD47 protein Human CD47 (Gln19-Pro139) (N-terminus) Accession # Q08722 IEGRMD Human IgG1 (Pro100-Lys330) (C-terminus)
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Structural Form: Disulfide-linked homodimer
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Gene Symbol: CD47
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Reconstitution: Reconstitute at 200 μg/mL in PBS.
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: CD47