R&D Systems™ Recombinant Human CD47 Mouse IgG2a Fc Chimera Protein

Description

Measured by its binding ability in a functional ELISA. When Recombinant Human SIRP alpha/CD172a Fc Chimera (Catalog # 4546-SA ) is immobilized at 0.2 μg/mL (100 μL/well), Recombinant Human CD47 Mouse IgG2a Fc Chimera (Catalog # 10673-CD) binds with an ED50 of 30.0-300 ng/mL.

Specifications

• Accession Number: Q08722.1
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Gene ID (Entrez): 961
• Molecular Weight (g/mol): M.W.-observed: 58-68 kDa, under reducing conditions., M.W.-theroretical: 41 kDa
• Quantity: 50 μg
• Source: Mouse myeloma cell line, NS0-derived human CD47 protein Human CD47 (Gln19-Pro139) (C-terminus) Accession # Q08722.1 IEGRMDP Mouse IgG2a (Glu98-Lys330) (N-terminus)
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Gene Alias: antigen identified by monoclonal 1D8, Antigenic surface determinant protein OA3, CD47, CD47 antigen, CD47 antigen (Rh-related antigen, integrin-associated signal transducer), CD47 glycoprotein, CD47 molecule, IAP, IAPintegrin associated protein, Integrin-associated protein, leukocyte surface antigen CD47, MER6, MER6integrin-associated signal transducer, OA3, Protein MER6, Rh-related antigen
• Conjugate: Unconjugated
• Reconstitution: Reconstitute at 500 μg/mL in PBS.
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: CD47