R&D Systems™ Recombinant Human CEACAM-1/CD66a Protein

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity

Measured by its ability to inhibit IL-2 secretion by HuT 78 human cutaneous T cell lymphoma cells Chen, C.J. and J.E. Shively (2004) J. Immunol. 172:3544. rhCEACAM-1 at concentrations higher than 1 μg/mL reduces anti-CD3 (immobilized at 10 μg/mL) induced IL-2 production by 60-70%. The ED50 for rhCEACAM-1 inhibition of anti-CD3 induced IL-2 production is 0.05-0.2 μg/mL.

Specifications

• Accession Number: P13688
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS.
• Gene ID (Entrez): 634
• Molecular Weight (g/mol): M.W. (Observed): 100-130 kDa, reducing conditions; M.W. (theoretical): 44.6 kDa
• Name: CEACAM-1/CD66a
• Quantity: 50 μg
• Source: Mouse myeloma cell line, NS0-derived human CEACAM-1/CD66a protein Gln35-Gly428, with a C-terminal 10-His tag
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Gene Symbol: CEACAM1
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Reconstitution: Reconstitute at 100 μg/mL in sterile PBS.
• Purity or Quality Grade: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: CEACAM1/CD66a