R&D Systems™ Recombinant Human Chitinase 3-like 2 Protein

Catalog No.	Quantity
5112CH050	50 μg

Catalog No. 5112CH050 Supplier R&D Systems™ Supplier No. 5112CH050

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility.

Measured by the ability of the immobilized protein to support the adhesion of FaDu human squamous cell carcinoma cells.

When 5 x 104 cells per well are added to rhCHI3L2 coated plates for 30 minutes at 37 °C, cell adhesion is enhanced in a dose dependent manner with typical ED50 range of 0.7-2.8 μg/mL.

Opitimal dilutions should be determined by each laboratory for each application.

Specifications

• Accession Number: Q15782
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS.
• Gene ID (Entrez): 1117
• Molecular Weight (g/mol): M.W. (Observed): 40 kDa, reducing conditions; M.W. (theoretical): 42.2 kDa
• Name: Chitinase 3-like 2
• Quantity: 50 μg
• Source: Mouse myeloma cell line, NS0-derived human Chitinase 3-like 2 protein Tyr27-Leu390, with a C-terminal 10-His tag
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.01 EU / 1 μg of the protein by the LAL method.
• Gene Symbol: CHI3L2
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Reconstitution: Reconstitute at 500 μg/mL in PBS.
• Purity or Quality Grade: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Protein: Chitinase 3-like 2