R&D Systems™ Recombinant Human CNTF Protein

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity

The Recombinant Human CNTF Protein is derived from E. coli. The Recombinant Human CNTF Protein has been validated for the following applications: Bioactivity.

Specifications

• Formulation: Lyophilized from a 0.2μm filtered solution in PBS, NaCl, EDTA, DTT and CHAPS with BSA as a carrier protein.
• Gene ID (Entrez): 1270
• Molecular Weight (g/mol): 22.8 kDa
• Name: CNTF
• Quantity: 10 μg
• Source: E. coli-derived human CNTF protein Ala2-Met200
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70° C as supplied. 3 months, 2 to 8° C under sterile conditions after reconstitution.
• Structural Form: This recombinant protein is prone to proteolytic degradation at the C-terminus, resulting in proteins with molecular masses of 22.2 - 22.8 kDa.
• Endotoxin Concentration: <0.1 EU per 1μg of the protein by the LAL method.
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Activity: Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED50 for this effect is 0.03-0.18 μg/mL. The proliferative effect can be enhanced up to 100-fold by the addition of Recombinant Human CNTF R alpha (Catalog # 303-CR ) .
• Purity or Quality Grade: 97%, by SDS-PAGE under reducing conditions and visualized by silver stain.