R&D Systems™ Recombinant Human DCC Protein

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility.

The Recombinant Human DCC Protein is derived from HEK293. The Recombinant Human DCC Protein has been validated for the following applications: Bioactivity.

Specifications

• Accession Number: P43146
• For Use With (Application): Bioactivity
• Gene ID (Entrez): 1630
• Molecular Weight (g/mol): Observed M.W.: 123-138kDa, reducing conditions, Theoretical M.W.: 119kDa
• Quantity: 50 μg
• Source: Human embryonic kidney cell,HEK293-derived human DCC protein Phe32-Asn1097,with a C-terminal 6-His tag
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70° C as supplied. 1 month, 2 to 8° C under sterile conditions after reconstitution. 3 months, -20 to -70° C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU per 1μg of the protein by the LAL method.
• Gene Alias: Colorectal cancer suppressor, CRC18, CRCR1, deleted in colorectal cancer protein, deleted in colorectal carcinoma, IGDCC1colorectal tumor suppressor, Immunoglobulin superfamily DCC subclass member 1, immunoglobulin superfamily, DCC subclass, member 1, netrin receptor DCC, Tumor suppressor protein DCC
• Common Name: DCC
• Gene Symbol: DCC
• Biological Activity: Measured by its binding ability in a functional ELISA. When Recombinant Human DCC was immobilized at 1μg/mL (100μL/well), it binds Recombinant Mouse Netrin-1. The concentration of Recombinant Mouse Netrin-1 that produces 50% of the optimal binding response is approximately 5-30ng/mL.
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Expression System: Human embryonic kidney cell, HEK293-derived Phe32-Asn1097, with a C-terminal 6-His tag
• Purity or Quality Grade: >90%, by SDS-PAGE with Silver Staining