R&D Systems™ Recombinant Human Dectin-1/CLEC7A Fc Chimera Protein

Description

HEK293 Expressed

Measured by its binding ability in a functional ELISA. When Recombinant Human Dectin-1/CLEC7A Fc Chimera (Catalog # 10364-DC) is immobilized at 0.25 μg/mL (100 μL/well), Biotinylated Laminarin (1, 3-beta-glucan) binds with an ED50 of 0.08-9.6 ng/mL.

Specifications

• Accession Number: Q9BXN2.1
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS.
• Gene ID (Entrez): 64581
• Molecular Weight (g/mol): Observed: 54-64 kDa, under reducing conditions; Theroretical: 47 kDa
• Quantity: 50 μg
• Source: Human embryonic kidney cell, HEK293-derived human Dectin-1/CLEC7A protein MD Human IgG1 (Pro100-Lys330) IEGR Human Dectin-1/CLEC7A (Thr66-Met247) Accession # Q9BXN2.1(N-terminus) (C-terminus)
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Conjugate: Unconjugated
• Reconstitution: Reconstitute at 500 μg/mL in PBS.
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
• Protein: Dectin-1/CLEC7A