R&D Systems™ Recombinant Human Dihydrolipoamide Dehydrogenase/DLD

Catalog No.	Quantity
8646DH050	50 μg

Catalog No. 8646DH050 Supplier R&D Systems™ Supplier No. 8646DH050

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility.

The Recombinant Human Dihydrolipoamide Dehydrogenase/DLD is derived from E. coli. The Recombinant Human Dihydrolipoamide Dehydrogenase/DLD has been validated for the following applications: Enzyme Activity.

Specifications

• Accession Number: P09622
• For Use With (Application): Enzyme Activity
• Gene ID (Entrez): 1738
• Molecular Weight (g/mol): Observed M.W.: 52-62kDa, reducing conditions, Theoretical M.W.: 52kDa
• Quantity: 50 μg
• Source: E. coli-derived human Dihydrolipoamide Dehydrogenase/DLD protein GGS HHHHHH GMASLENLYFQ Human DLD (Ala36-Phe509) Accession &Num; P09622 N-terminus C-terminusAla36-Phe509,with N-terminal GGS-6-His tag and GMASLENLYFQ insertion
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70° C as supplied. 3 months, -20 to -70° C under sterile conditions after opening.
• Endotoxin Concentration: <1.0 EU per 1μg of the protein by the LAL method.
• Gene Alias: 2-oxo-glutarate complex, branched chain keto acid dehydrogenase complex), branched chain keto acid dehydrogenase complex, Diaphorase, dihydrolipoamide dehydrogenase (E3 component of pyruvate dehydrogenase complex, dihydrolipoamide dehydrogenasemitochondrial, DLD, DLDD, DLDH, E3, E3 component of pyruvate dehydrogenase complex, 2-oxo-glutarate complex, EC 1.8.1, EC 1.8.1.4, GCSL, Glycine cleavage system L protein, glycine cleavage system protein L, LAD, Lipoamide Dehydrogenase, lipoamide reductase, lipoyl dehydrogenase, PHE3
• Common Name: Dihydrolipoamide Dehydrogenase/DLD
• Gene Symbol: DLD
• Biological Activity: Measured by its ability to produce NADH during the oxidation of lipoic acid. The specific activity is >5,000 pmol/min/μg, as measured under the described conditions. See Activity Assay Protocol on www.RnDSystems.com.
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Expression System: E. coli-derived GGS, N-terminus; HHHHHH; GMASLENLYFQ; Human DLD (Ala36-Phe509) Accession # P09622, C-terminus (Ala36 - Phe509, with N-terminal GGS-6-His tag and GMASLENLYFQ insertion)
• Purity or Quality Grade: >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie™ Blue stain at 5μg per lane