R&DSystems™ Recombinant Human Dkk-1 Protein, CF

Catalog No.	Quantity
11-495-DK500	500 μg
11-495-DK100	100 μg
11-495-DK050	50 μg
11-495-DK020	20 μg
11-495-DK01M	1 mg
11-495-DK250	250 μg

Catalog No. 11-495-DK500 Supplier R&DSystems™ Supplier No. 11495DK500

Description

Recombinant Protein

Measured by its ability to inhibit Wnt induced TCF reporter activity in HEK293 human embryonic kidney cells. The ED50 for this effect is 10.0-250 ng/mL.

Specifications

• Accession Number: O94907.1
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Gene ID (Entrez): 22943
• Molecular Weight (g/mol): M.W.-observed: 33-40 kDa, under reducing conditions., M.W.-theroretical: 26 kDa
• Quantity: 500 μg
• Source: Chinese Hamster Ovary cell line, CHO-derived human Dkk-1 protein Thr32-His266
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU per 1 μg of the protein by the LAL method.
• Gene Alias: dickkopf (Xenopus laevis) homolog 1, dickkopf homolog 1 (Xenopus laevis), dickkopf related protein-1, Dickkopf-1, dickkopf-related protein 1, Dkk1, DKK-1, hDkk-1, SKdickkopf-1 like
• Conjugate: Unconjugated
• Reconstitution: Reconstitute the 20 μg size at 200 μg/mL in PBS. Reconstitute all other sizes at 500 μg/mL in PBS.
• Purity or Quality Grade: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: Dkk-1