R&D Systems™ Recombinant Human DNAM-1/CD226 His-tag Avi-tag Protein

Catalog No.	Quantity
AVI9298050	50 μg

Catalog No. AVI9298050 Supplier R&D Systems™ Supplier No. AVI9298050

Description

Biotinylated

The biotin to protein ratio is greater than 0.7 as determined by the HABA assay. Measured by its binding ability in a functional ELISA. When 250 ng/mL of Biotinylated Recombinant Human DNAM-1/CD226 His-tag Avi-tag (AVI9298) protein is immobilized onto Streptavidin coated plate (Catalog # CP004 ), it binds to Recombinant Human CD155/PVR Fc Chimera (Catalog # 9174-CD ) with an ED50 of 20-100 ng/mL.

Specifications

• Accession Number: Q15762.2
• For Use With (Application): Bioactivity
• Formulation: Supplied as a 0.2 μm filtered solution in PBS.
• Gene ID (Entrez): 10666
• Molecular Weight (g/mol): M.W. (Observed): 35-60 kDa, under reducing conditions; M.W. (theoretical): 29 kDa
• Purification Method: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Quantity: 50 μg
• Source: Human embryonic kidney cell, HEK293-derived human DNAM-1/CD226 protein Human DNAM-1 (Glu19-Asn247) (N-terminus) Accession # Q15762.2 HHHHHH Avi-tag (C-terminus)
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -70 °C as supplied. 1 month, 2°C to 8°C under sterile conditions after opening. 3 months, -20°C to -70°C under sterile conditions after opening.
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Conjugate: Biotin
• Buffer: Supplied as a 0.2 μm filtered solution in PBS
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: DNAM-1/CD226