R&D Systems™ Recombinant Human E-Cadherin Protein

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity

The Recombinant Human E-Cadherin Protein is derived from HEK293. The Recombinant Human E-Cadherin Protein has been validated for the following applications: Bioactivity.

Specifications

• Accession Number: P12830
• For Use With (Application): Bioactivity
• Gene ID (Entrez): 999
• Molecular Weight (g/mol): Observed M.W.: 74-92kDa, reducing conditions, Theoretical M.W.: 61kDa
• Quantity: 50 μg
• Source: Human embryonic kidney cell,HEK293-derived human E-Cadherin protein Asp155-Ile707,with a C-terminal 6-His tag
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70° C as supplied. 1 month, 2 to 8° C under sterile conditions after reconstitution. 3 months, -20 to -70° C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU per 1μg of the protein by the LAL method.
• Gene Alias: Arc-1, CAD1, cadherin 1, E-cadherin (epithelial), cadherin 1, type 1, E-cadherin (epithelial), cadherin-1, calcium-dependent adhesion protein, epithelial, CAM 120/80, CD324, CD324 antigen, CDH1, CDHE, cell-CAM 120/80, Cell-CAM120/80, ECAD, E-cadherin, ECadherin, Epithelial cadherin, LCAM, L-CAM, UVOE-Cadherin, uvomorulin
• Common Name: E-Cadherin
• Gene Symbol: CDH1
• Biological Activity: Measured by the ability of the immobilized protein to support the adhesion of the MCF-7 human breast cancer cells. The ED50 for this effect is typically 0.2-1μg/mL
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Expression System: Human embryonic kidney cell, HEK293-derived Asp155-Ile707, with a C-terminal 6-His tag
• Purity or Quality Grade: >95%, by SDS-PAGE with Silver Staining