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R&D Systems™ Recombinant Human E-Selectin/CD62E His-tag Protein

Catalog No. 10258ES100
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100 μg
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Catalog No. Quantity
10-258-ES100 100 μg
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Catalog No. 10-258-ES100 Supplier R&D Systems™ Supplier No. 10258ES100
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Description

Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells.

ED50 for this effect is 0.2 to 1 μg/mL

Specifications

Accession Number P16581
For Use With (Application) Bioactivity
Gene ID (Entrez) 6401
Molecular Weight (g/mol) 84 to 109 kDa, under reducing conditions
Quantity 100 μg
Source Chinese Hamster Ovary cell line, CHO-derived human E-Selectin/CD62E protein Trp22-Pro556, with a C-terminal 6-His tag
Storage Requirements Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Endotoxin Concentration < 0.10 EU per 1 μg of the protein by the LAL method
Conjugate Unconjugated
Species Human
Reconstitution Reconstitute at 500 μg/mL in PBS
Buffer Lyophilized from a 0.2 μm filtered solution in PBS
Purity or Quality Grade > 95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining
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