R&D Systems™ Recombinant Human EGF GMP Protein, CF

Catalog No.	Quantity
23-6GM-P200	200 μg

Catalog No. 23-6GM-P200 Supplier R&D Systems™ Supplier No. 236GMP200

Description

Animal-Free.

Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. Rubin, J.S. et al. (1991) Proc. Natl. Acad. Sci. USA 88:415. The ED50 for this effect is 20-100 pg/mL. The specific activity of Recombinant Human EGF is >8.0 x 105 IU/mg, which is calibrated against the human EGF WHO International Standard (NIBSC code: 91/530).

Specifications

• Accession Number: P01133.2
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS.
• Gene ID (Entrez): 1950
• Molecular Weight (g/mol): Observed: 6 kDa, reducing conditions, Observed: 6 kDa
• Quantity: 200 μg
• Source: E. coli-derived human EGF protein Asn971-Arg1023, with an N-terminal Met Produced using non-animal reagents in an animal-free laboratory. Manufactured and tested under cGMP guidelines.
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. A minimum of 12 months when stored at ≤ -20°C as supplied. Refer to lot specific COA for the Use by Date. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, ≤ -20°C under sterile conditions after reconstitution.
• Regulatory Status: GMP
• Endotoxin Concentration: <0.10 EU per 1 μg of the protein by the LAL method.
• Gene Alias: beta-urogastrone, epidermal growth factor, epidermal growth factor (beta-urogastrone), hEGF, HOMG4, pro-epidermal growth factor, URG, Urogastrone
• Gene Symbol: EGF
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Reconstitution: Reconstitute at 200 μg/mL in PBS.
• Purity or Quality Grade: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain. The result of the major peak from mass spectrometry analysis is 6347 Da, which corresponds to the calculated molecular mass of 6353 Da
• Protein: EGF