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R&D Systems™ Recombinant Human EGF Protein
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Quantity:
1 mg
200 μg
Description
Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. Rubin, J.S. et al. (1991) Proc. Natl. Acad. Sci. USA 88:415. The ED50 for this effect is 20-100 pg/mL.
Specifications
Specifications
Accession Number | P01133 |
For Use With (Application) | Bioactivity |
Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
Gene ID (Entrez) | 1950 |
Molecular Weight (g/mol) | M.W. (Observed): 6 kDa, reducing conditions; M.W. (theoretical): 6 kDa |
Name | EGF |
Quantity | 200 μg |
Source | E. coli-derived human EGF protein Asn971-Arg1023, with an N-terminal Met |
Storage Requirements | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution. |
Endotoxin Concentration | <0.10 EU / 1 μg of the protein by the LAL method. |
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