R&D Systems™ Recombinant Human EGFR Fc Chimera Alexa Fluor™ 488 Protein

Description

Measured by flow cytometry for its ability to bind anti-human EGFR Monoclonal Antibody conjugated beads. The concentration of Recombinant Human EGFR Fc-tag Alexa Fluor™ 488 (Catalog # AFG344) that produces 50% of the binding response is 8.00-120 ng/mL.

Specifications

• Accession Number: CAA25240.1
• For Use With (Application): Bioactivity
• Formulation: Supplied as a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Gene ID (Entrez): 1956
• Molecular Weight (g/mol): M.W.-observed: 118-134 kDa, under reducing conditions., M.W.-theroretical: 95 kDa (monomer)
• Quantity: 20 μg
• Source: Mouse myeloma cell line, NS0-derived human EGFR protein Human EGFR (Leu25-Ser645) (C-terminus) Accession # CAA25240.1 IEGRMD Human IgG1 (Pro100-Lys330) (N-terminus)
• Storage Requirements: Protect from light. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after opening. 3 months, -20°C to -70°C under sterile conditions after opening.
• Endotoxin Concentration: <1.0 EU / 1 μg of the protein by the LAL method.
• Gene Alias: avian erythroblastic leukemia viral (v-erb-b) oncogene homolog, cell growth inhibiting protein 40, cell proliferation-inducing protein 61, EC 2.7.10, EC 2.7.10.1, EGF R, epidermal growth factor receptor, epidermal growth factor receptor (avian erythroblastic leukemia viral (v-erb-b)oncogene homolog), ErbB, ErbB1, ERBB1PIG61, HER1, HER-1, mENA, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1
• Conjugate: Alexa Fluor 488
• Purity or Quality Grade: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: EGFR