R&D Systems™ Recombinant Human Elongin B/Elongin C/VHL/CUL2/RBX1

Description

Typical protein concentration for use in vitro will depend on experimental conditions.

Typical protein concentration for use in vitro will depend on experimental conditions.

Specifications

• Accession Number: P40337.2, Q15370.1, Q15369.1, Q13617.2, P62877.1
• For Use With (Application): Bioactivity
• Formulation: Supplied as a 0.2 μm filtered solution in HEPES, NaCl, DTT and Glycerol.
• Molecular Weight (g/mol): M.W. (theoretical): 25 kDa (VHL), 13.1 kDa (ELOB), 12.8 kDa (ELOC), 93 kDa (CUL2), 12 kDa (RB x 1)
• Quantity: 25 μg
• Source: Spodoptera frugiperda, Sf 21 (baculovirus)-derived human VHL/ELOB/ELOC/CUL2/RB x 1 Complex protein Met231 - Asp443 (VHL), Met1 - Gln118 (ELOB), Met1 - Cys112 (ELOC), Met1 - Ala 745 with a N-terminal 10-His tag Sumo tag (CUL2), Met1 - His108 (RB x 1)
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -70°C as supplied. 3 months, -70°C under sterile conditions after opening.
• Conjugate: Unconjugated
• Species: Human
• Buffer: X mg/mL (X μm) in 50 m, HEPES pH 7.5, 200 mm NaCl, 10% (v/v) Glycerol, 2 mm DTT
• Purity or Quality Grade: >90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie™ Blue stain.
• Protein: VHL/ELOB/ELOC/CUL2/RBX1 Complex