R&D Systems™ Recombinant Human ErbB3/Her3 His-tag Protein

Catalog No.	Quantity
10-368-RB050	50 μg

Catalog No. 10-368-RB050 Supplier R&D Systems™ Supplier No. 10368RB050

Description

Measured by its ability to inhibit the biological activity of Neuregulin-1-beta 1 on MCF-7 human breast cancer cells.

Karey, K.P. et al. (1988) Cancer Research 48:4083. The ED50 for this effect is 0.3-3 μg/mL in the presence of 10 ng/mL Recombinant Human NRG1-beta 1/HRG1-beta 1 Extracellular Domain (Catalog # 377-HB ).

Specifications

• Accession Number: P21860.1
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS.
• Gene ID (Entrez): 2065
• Molecular Weight (g/mol): Observed: 73-83 kDa, under reducing conditions; Theroretical: 61 kDa
• Quantity: 50 μg
• Source: Human embryonic kidney cell, HEK293-derived human ErbB3/Her3 protein Human ErbB3/Her3 (Ser20-Gly538) Accession # P21860-1(N-terminus) YSKGSQSRMGGGGALQWNCSGGIQ 6-His tag (C-terminus)
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Endotoxin Concentration: <1.0 EU / 1 μg of the protein by the LAL method.
• Conjugate: Unconjugated
• Reconstitution: Reconstitute at 500 μg/mL in PBS.
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
• Protein: ErbB3/Her3