R&D Systems™ Recombinant Human Erythropoietin/EPO Protein

Description

Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED50 for this effect is 0.075-0.750 ng/mL.

Specifications

• Accession Number: CAA26094.1
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Molecular Weight (g/mol): M.W.-observed: 33-41 kDa, under reducing conditions., M.W.-theroretical: 21 kDa
• Quantity: 10 μg
• Source: Chinese Hamster Ovary cell line, CHO-derived human Erythropoietin/EPO protein Ala28-Arg193
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Conjugate: Unconjugated
• Reconstitution: Reconstitute at 100-500 μg/mL in PBS.
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.