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R&D Systems™ Recombinant Human Exostosin 1/2 Heterodimer Protein

Catalog No. 8567GT020
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20 μg
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Catalog No. Quantity
85-67G-T020 20 μg
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Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Enzyme Activity

Measured by its ability to transfer GlcNAc and GlcA from donor substrates to heparan sulfate. The specific activity is >225 pmol/min/μg, as measured under the described conditions.

Specifications

Accession Number Q16394 (EXT1) & Q93063 (EXT2)
For Use With (Application) Enzyme Activity
Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl and Glycerol.
Molecular Weight (g/mol) M.W. (Observed): 69-85 kDa, reducing conditions; M.W. (theoretical): 84 kDa (EXT1) & 77 kDa (EXT2)
Quantity 20 μg
Source Chinese Hamster Ovary cell line, CHO-derived human Exostosin 1/2 Heterodimer protein Arg29-Leu746 with a C-terminal 2-His and HA tag (EXT1) & Ser53-Leu718 with a C-terminal 6-His tag (EXT2)
Storage Requirements Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20°C to -70°C as supplied. 3 months, -20°C to -70°C under sterile conditions after opening.
Endotoxin Concentration <1.0 EU / 1 μg of the protein by the LAL method.
Common Name Exostosin 1/2 Heterodimer
Biological Activity Measured by its ability to transfer GlcNAc and GlcA from donor substrates to heparan sulfate. The specific activity is >225 pmol/min/μg, as measured under the described conditions.
Conjugate Unconjugated
Species Human
Recombinant Recombinant
Expression System Chinese Hamster Ovary cell line, CHO-derived Arg29-Leu746 with a C-terminal 2-His and HA tag (EXT1) & Ser53-Leu718 with a C-terminal 6-His tag (EXT2)
Purity or Quality Grade >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie™ Blue stain (at 5 μg / lane.)
Protein Exostosin 1/2 Heterodimer
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