R&D Systems™ Recombinant Human Fc epsilon RI alpha Avi His Protein

Catalog No.	Quantity
AVI6678050	50 μg

Catalog No. AVI6678050 Supplier R&D Systems™ Supplier No. AVI6678050

Description

Biotinylated Avi-tag His-tag

Measured by its binding ability in a functional ELISA. When Human IgE is immobilized at 1.00 μg/mL (100 μL/well), Biotinylated Recombinant Human Fc epsilon RI alpha Avi-tag His-tag binds with an ED50 of 0.300-1.80 ng/mL.

Specifications

• Accession Number: P12319.1
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Molecular Weight (g/mol): MolecularWeight-observed: 50-60 kDa, under reducing conditions., MolecularWeight-theroretical: 25 kDa
• Quantity: 50 μg
• Source: Chinese Hamster Ovary cell line, CHO-derived human Fc epsilon RI alpha protein Human FCER-1 alpha (Val26-Gln205) (N-terminus) Accession # P12319.1 Avi-tag 6-His tag (C-terminus)
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Conjugate: Biotin
• Reconstitution: Reconstitute at 500 μg/mL in PBS.
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: Fc epsilon RI alpha