R&D Systems™ Recombinant Human Fc gamma RIIB/CD32b Avi-tag Protein

Catalog No.	Quantity
AVI1875050	50 μg

Catalog No. AVI1875050 Supplier R&D Systems™ Supplier No. AVI1875050

Description

Biotinylated

Measured by its binding ability in a functional ELISA. When Normal Human IgG is immobilized at 10 μg/mL (100 μL/well), the concentration of Biotinylated Recombinant Human Fc gamma RIIB/C (CD32b/c) His-tag Avi-tag (Catalog # AVI1875) that produces 50% of the optimal binding response is found to be approximately 0.5-3.0 μg/mL.

Specifications

• Accession Number: P31995.1
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Gene ID (Entrez): 2213
• Molecular Weight (g/mol): Observed: 25-36 kDa, under reducing conditions; Theroretical: 22 kDa
• Quantity: 50 μg
• Source: Human embryonic kidney cell, HEK293-derived human Fc gamma RIIB/C (CD32b/c) protein Human Fc gamma RIIB/C (CD32b/c) (Ala46-Pro217) Accession # P31995.1(N-terminus) HHHHHH Avi-tag (C-terminus)
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Conjugate: Biotin
• Reconstitution: Reconstitute at 500 μg/mL in PBS.
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
• Protein: Fc gamma RIIB/C (CD32b/c)