R&D Systems™ Recombinant Human Fc gamma RIIIA (F176) His Avi Protein

Description

The Recombinant Human Fc gamma RIIIA (F176) His Avi Protein is derived from HEK293

• Recombinant Protein
• Carrier Free (CF)
• The carrier free version does not contain Bovine Serum Albumin (BSA)

Specifications

• Accession Number: P08637-1
• For Use With (Application): Bioactivity
• Gene ID (Entrez): 2214
• Molecular Weight (g/mol): 25 kDa
• Purification Method: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining
• Quantity: 50 μg
• Source:

  Human embryonic kidney cell, HEK293-derived human Fc gamma RIIIA/CD16a protein

  Human Fc gamma RIIIA (F176) (Gly17-Gln208) Accession # P08637-1HHHHHHHAvi-tagN-terminus C-terminus

• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 month, 2 to 8°C under sterile conditions after reconstitution. 3 months, -20 to -70°C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU per 1 μg of the protein by the LAL method
• Conjugate: Biotin
• Reconstitution: Reconstitute at 200 μg/mL in PBS
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose
• Activity: The biotin to protein ratio is greater than 0.7 as determined by the HABA assay. Measured by its binding ability in a functional ELISA. When Human IgG is immobilized at 5 μg/mL (100 μL/well), Biotinylated Recombinant Human Fc gamma RIIIA/CD16a (F176) His-tag Avi-tag (Catalog # AVI8894) binds with an ED50 of 50-300 ng/mL.