R&D Systems™ Recombinant Human FGF basic/FGF2/bFGF, 145 aa TC Grade

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity

This protein was used at 20-50 ng/mL to support a 100-fold expansion of rat cortical neural stem cells or mouse cortical stem cells. Cells were cultured in N2 plus medium and supplemented with fresh FGF basic/FGF2/bFGF every day. Medium was changed every other day and cells were split every 2-4 days upon becoming 70% confluent. Measured in a cell proliferation assay using NR6R-3T3 mouse fibroblast cells. Raines, E.W. et al. (1985) Methods Enzymol. 109:749. The ED50 for this effect is 0.3-3 ng/mL.

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Specifications

• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2μm filtered solution in Tris-HCl and NaCl.
• Gene ID (Entrez): 2247
• Molecular Weight (g/mol): 16 kDa
• Name: FGF basic 145 aa, Tissue Culture Grade
• Quantity: 1 mg
• Source: E. coli-derived human FGF basic/FGF2/bFGF protein Ala144-Ser288
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70° C as supplied. 1 month, 2 to 8° C under sterile conditions after reconstitution. 3 months, -20 to -70° C under sterile conditions after reconstitution.
• Gene Alias: Basic fibroblast growth factor, basic fibroblast growth factor bFGF, bFGF, FGF2, FGF-2, FGFBprostatropin, fibroblast growth factor 2 (basic), HBGF-2, heparin-binding growth factor 2, Prostatropin
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Reconstitution: Reconstitute at 0.5-1.0 mg/mL in sterile PBS.