R&D Systems™ Recombinant Human FGF basic/FGF2/bFGF (146 aa) Protein

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity

Measured in a cell proliferation assay using NR6R-3T3 mouse fibroblast cells. Raines, E.W. et al. (1985) Methods Enzymol. 109:749. The ED50 for this effect is 0.1-0.6 ng/mL.

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Specifications

• Formulation: Lyophilized from a 0.2μm filtered solution in Tris-HCl and NaCl with BSA as a carrier protein.
• Gene ID (Entrez): 2247
• Molecular Weight (g/mol): 16.5 kDa
• Name: FGF basic 146 aa
• Quantity: 25 μg
• Source: E. coli-derived human FGF basic/FGF2/bFGF protein Pro143-Ser288,with an N-terminal Ala
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70° C as supplied. 1 month, 2 to 8° C under sterile conditions after reconstitution. 3 months, -20 to -70° C under sterile conditions after reconstitution.
• Gene Alias: Basic fibroblast growth factor, basic fibroblast growth factor bFGF, bFGF, FGF2, FGF-2, FGFBprostatropin, fibroblast growth factor 2 (basic), HBGF-2, heparin-binding growth factor 2, Prostatropin
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Reconstitution: Reconstitute at 100-250μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
• Purity or Quality Grade: 97%, by SDS-PAGE under reducing conditions and visualized by silver stain.