R&D Systems™ Recombinant Human FGF basic/FGF2/bFGF, Animal-Free Protein

Description

Also available, GMP FGF basic/FGF2/bFGF Protein, Catalog # 3718-GMP! Animal-free and manufactured under GMP guidelines for therapeutic manufacturing applications.

Measured in a cell proliferation assay using NR6R-3T3 mouse fibroblast cells. Raines, E.W. et al. (1985) Methods Enzymol. 109:749. The ED50 for this effect is 0.1-0.6 ng/mL. The specific activity of Recombinant Human FGF basic/FGF2/bFGF is approximately 800 IU/μg, which is calibrated against human FGF basic/FGF2/bFGF WHO International Standard (NIBSC code: 90/712).

Specifications

• Accession Number: NP_001997
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in Tris-HCl and NaCl.
• Gene ID (Entrez): 2247
• Molecular Weight (g/mol): M.W.-theoretical: 16 kDa
• Quantity: 25 μg
• Source: E. coli-derived human FGF basic/FGF2/bFGF protein Pro143-Ser288 Produced using non-animal reagents in an animal-free laboratory.
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.01 EU / 1 μg of the protein by the LAL method.
• Gene Alias: Basic fibroblast growth factor, basic fibroblast growth factor bFGF, bFGF, FGF2, FGF-2, FGFBprostatropin, fibroblast growth factor 2 (basic), HBGF-2, heparin-binding growth factor 2, Prostatropin
• Conjugate: Unconjugated
• Reconstitution: Reconstitute at 100 μg/mL in PBS.
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: FGF basic/FGF2/bFGF