R&D Systems™ Recombinant Human FGF basic Heat Stable Protein, Animal-Free

Catalog No.	Quantity
RD10000340	25 μg
RD10000339	1 mg

Catalog No. RD10000340 Supplier R&D Systems™ Supplier No. BTFGFBHSAFL025

Description

Recombinant Protein

Measured in a cell proliferation assay using NR6R-3T3 mouse fibroblast cells. Raines, E.W. et al. (1985) Methods Enzymol. 109:749. The ED50 for this effect is 0.0500-0.600 ng/mL. The specific activity of Animal-Free™ Recombinant Human FGF basic Heat Stable is >1.60 x 106 IU/mg, which is calibrated against the human FGF basic/FGF2 WHO International Standard (NIBSC code: 90/712).

Specifications

• Accession Number: NP_001997.5
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in HEPES and Sodium Sulfate with Trehalose.
• Gene ID (Entrez): 2247
• Molecular Weight (g/mol): MolecularWeight-observed: 16-19 kDa, under reducing conditions, MolecularWeight-theroretical: 17 kDa
• Quantity: 25 μg
• Source: E. coli-derived human FGF basic/FGF2/bFGF protein Ala135-Ser288 (with modifications) Produced using non-animal reagents in an animal-free laboratory.
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU per 1 μg of the protein by the LAL method.
• Gene Alias: Basic fibroblast growth factor, basic fibroblast growth factor bFGF, bFGF, FGF2, FGF-2, FGFBprostatropin, fibroblast growth factor 2 (basic), HBGF-2, heparin-binding growth factor 2, Prostatropin
• Conjugate: Unconjugated
• Reconstitution: Reconstitute the 25 μg size at 250 μg/mL in sterile water. Reconstitute all other sizes at 500 μg/mL in sterile water.
• Protein: FGF basic/FGF2/bFGF