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R&DSystems™ Recombinant Human FGF basic Heat Stable Protein, CF

Catalog No. BTFGFBHS100
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Catalog No. Quantity
BTFGFBHS100 100 μg
BTFGFBHS500 500 μg
BTFGFBHS050 50 μg
BTFGFBHS010 10 μg
BTFGFBHS01M 1 mg
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Catalog No. BTFGFBHS100 Supplier R&DSystems™ Supplier No. BTFGFBHS100
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Description

Recombinant Protein

Measured in a cell proliferation assay using NR6R-3T3 mouse fibroblast cells. Raines, E.W. et al. (1985) Methods Enzymol. 109:749. The ED50 for this effect is 0.0500-0.600 ng/mL.

Specifications

Accession Number NP_001997.5
For Use With (Application) Bioactivity
Formulation Lyophilized from a 0.2 μm filtered solution in HEPES and Sodium Sulfate with Trehalose.
Gene ID (Entrez) 2247
Molecular Weight (g/mol) M.W.-observed: 16-19 kDa, under reducing conditions., M.W.-theroretical: 17 kDa
Quantity 100 μg
Source E. coli-derived human FGF basic/FGF2/bFGF protein Ala135-Ser288 (with modifications)
Storage Requirements Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Endotoxin Concentration <0.10 EU per 1 μg of the protein by the LAL method.
Gene Alias Basic fibroblast growth factor, basic fibroblast growth factor bFGF, bFGF, FGF2, FGF-2, FGFBprostatropin, fibroblast growth factor 2 (basic), HBGF-2, heparin-binding growth factor 2, Prostatropin
Conjugate Unconjugated
Reconstitution Reconstitute 10 μg size at 100 μg/mL in sterile deionized water. Reconstitute all the other sizes at 500 μg/mL in sterile deionized water.
Protein FGF basic/FGF2/bFGF
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