R&D Systems™ Recombinant Human Fibronectin Fragment 3 Protein

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity

Measured by the ability of the immobilized protein to support the adhesion of B16-F1 mouse melanoma cells. When 5 x 104 cells/well are added to Recombinant Human Fibronectin Fragment 3/amino acids 1266-1908 coated plates (1 μg/mL with 100 μL/well), approximately 50-75% will adhere after 30-60 minutes at 37 °C. Optimal concentration depends on cell type as well as the application or research objective.

Specifications

• Accession Number: NP_997647.2
• Formulation: Lyophilized from a 0.2μm filtered solution in PBS and Tween™ 20.
• Gene ID (Entrez): 2335
• Molecular Weight (g/mol): 70.1 kDa
• Name: Fibronectin Fragment 3
• Quantity: 50 μg
• Source: Mouse myeloma cell line, NS0-derived human Fibronectin protein Glu1266-Pro1908 (Met1874Thr), with a C-terminal 6-His tag
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70° C as supplied. 1 month, 2 to 8° C under sterile conditions after reconstitution. 3 months, -20 to -70° C under sterile conditions after reconstitution.
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Purity or Quality Grade: 90%, by SDS-PAGE under reducing conditions and visualized by silver stain.