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R&D Systems™ Recombinant Human Fibronectin Fragment 3 Protein

Catalog No. 3938FN050
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Quantity:
50 μg
This item is not returnable. View return policy
This item is not returnable. View return policy

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity

Measured by the ability of the immobilized protein to support the adhesion of B16-F1 mouse melanoma cells. When 5 x 104 cells/well are added to Recombinant Human Fibronectin Fragment 3/amino acids 1266-1908 coated plates (1 μg/mL with 100 μL/well), approximately 50-75% will adhere after 30-60 minutes at 37 °C. Optimal concentration depends on cell type as well as the application or research objective.

Specifications

Accession Number NP_997647.2
Formulation Lyophilized from a 0.2μm filtered solution in PBS and Tween™ 20.
Gene ID (Entrez) 2335
Molecular Weight (g/mol) 70.1 kDa
Name Fibronectin Fragment 3
Quantity 50 μg
Source Mouse myeloma cell line, NS0-derived human Fibronectin protein Glu1266-Pro1908 (Met1874Thr), with a C-terminal 6-His tag
Storage Requirements Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70° C as supplied. 1 month, 2 to 8° C under sterile conditions after reconstitution. 3 months, -20 to -70° C under sterile conditions after reconstitution.
Conjugate Unconjugated
Recombinant Recombinant
Purity or Quality Grade 90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
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