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R&D Systems™ Recombinant Human Fibronectin Fragment 3 Protein
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Quantity:
50 μg
Description
Measured by the ability of the immobilized protein to support the adhesion of B16-F1 mouse melanoma cells. When 5 x 104 cells/well are added to Recombinant Human Fibronectin Fragment 3/amino acids 1266-1908 coated plates (1 μg/mL with 100 μL/well), approximately 50-75% will adhere after 30-60 minutes at 37 °C. Optimal concentration depends on cell type as well as the application or research objective.
Specifications
Specifications
Accession Number | NP_997647.2 |
Formulation | Lyophilized from a 0.2μm filtered solution in PBS and Tween™ 20. |
Gene ID (Entrez) | 2335 |
Molecular Weight (g/mol) | 70.1 kDa |
Name | Fibronectin Fragment 3 |
Quantity | 50 μg |
Source | Mouse myeloma cell line, NS0-derived human Fibronectin protein Glu1266-Pro1908 (Met1874Thr), with a C-terminal 6-His tag |
Storage Requirements | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70° C as supplied. 1 month, 2 to 8° C under sterile conditions after reconstitution. 3 months, -20 to -70° C under sterile conditions after reconstitution. |
Conjugate | Unconjugated |
Recombinant | Recombinant |
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