R&D Systems™ Recombinant Human Flt-3 Ligand/FLT3L GMP Protein, CF

Catalog No.	Quantity
RD10008962	50 μg

Catalog No. RD10008962 Supplier R&D Systems™ Supplier No. BTFT3LGMP050

Description

Recombinant Protein

Measured in a cell proliferation assay using OCI-AML5 acute myeloid leukemia cells. The ED50 for this effect is 0.200-2.00 ng/mL. The specific activity of Recombinant Human Flt-3 Ligand/FLT3L is >5.00 x 105 units/mg, which is calibrated against the human Flt-3 Ligand WHO standard (NIBSC code: 96/532).

Specifications

• Accession Number: P49771.1
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Gene ID (Entrez): 2323
• Molecular Weight (g/mol): Observed: 17 kDa, under reducing conditions., Observed: 18 kDa
• Quantity: 50 μg
• Source: E. coli-derived human Flt-3 Ligand/FLT3L protein Thr27 - Ala181 Produced using non-animal reagents in an animal-free laboratory. Manufactured and tested under cGMP guidelines.
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. A minimum of 12 months when stored at ≤ -20°C as supplied. Refer to lot specific COA for the Use by Date. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, ≤ -20°C under sterile conditions after reconstitution.
• Regulatory Status: GMP
• Endotoxin Concentration: <0.10 EU per 1 μg of the protein by the LAL method.
• Gene Alias: FL, FLG3L, Flt3 ligand, Flt3L, FLT3LG, fms-related tyrosine kinase 3 ligand, SL cytokine
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Reconstitution: Reconstitute at 500 μg/mL in PBS.
• Purity or Quality Grade: >97%, by SDS-PAGE with quantitative densitometry by Coomassie™ Blue Staining. The molecular weight by mass spectrometry is 17731 Da ± 50 Da, with a second mass of 17600 Da ± 50 Da also present
• Protein: Flt-3 Ligand/FLT3L