R&D Systems™ Recombinant Human Flt-3 Ligand/FLT3L (HEK293) Protein

Description

Measured in a cell proliferation assay using BaF3 mouse pro-B cells transfected with mouse Flt-3. The ED50 for this effect is 0.150-1.50 ng/mL.

Specifications

• Accession Number: AAA17999.1
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Gene ID (Entrez): 2323
• Molecular Weight (g/mol): M.W.-observed: 22-31 kDa, under reducing conditions., M.W.-theroretical: 18 kDa
• Quantity: 10 μg
• Source: Human embryonic kidney cell, HEK293-derived human Flt-3 Ligand/FLT3L protein Thr27-Pro185
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Gene Alias: FL, FLG3L, Flt3 ligand, Flt3L, FLT3LG, fms-related tyrosine kinase 3 ligand, SL cytokine
• Conjugate: Unconjugated
• Reconstitution: Reconstitute the 10 μg size at 100 μg/mL in PBS. Reconstitute all other sizes (at 250 μg/mL in PBS.)
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: Flt-3 Ligand/FLT3L