R&D Systems™ Recombinant Human Galectin-9 Protein

Description

Includes

For Research Use Only

HEK293 rh Galectin-9 (Catalog # 9064-GA) is also available. TIM-3 binding is weaker than the E. coli version!

Measured by its ability to induce apoptosis of Jurkat human acute T cell leukemia cells. Lu, L.H. et al. (2007) J. Biochem. 141:157. The ED50 for this effect is 1-5 μg/mL. Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human Galectin-9 at 500 ng/mL can bind Recombinant Human TIM-3 Fc Chimera (Catalog # 2365-TM ) with an apparent Kd <30 nM.

Order Info

Ships at ambient temperature.

Specifications

• Accession Number: BAA31542
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in MOPS, NaCl, EDTA, DTT and Trehalose.
• Gene ID (Entrez): 3965
• Molecular Weight (g/mol): M.W. (Observed): 34 kDa, reducing conditions; M.W. (theoretical): 35.8 kDa
• Quantity: 1 mg
• Source: E. coli-derived human Galectin-9 protein Ala2-Thr323
• Storage Requirements: 12 months from date of receipt, <= -20 °C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, <= -20 °C under sterile conditions after reconstitution.
• Regulatory Status: RUO
• Structural Form: Monomer
• Endotoxin Concentration: <1.0 EU / 1 μg of the protein by the LAL method.
• Biological Activity: 2.5 to 12.5μg/mL
• Product Type: Recombinant protein
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Reconstitution: Reconstitute at 100 μg/mL aq. soln..
• Purity or Quality Grade: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Protein: Galectin-9

For Research Use Only.