R&D Systems™ Recombinant Human GDNF GMP Protein, CF

Catalog No.	Quantity
RD10008952	50 μg

Catalog No. RD10008952 Supplier R&D Systems™ Supplier No. BTGDNFGMP050

Description

Recombinant Protein

Measured in a cell proliferation assay using SH-SY5Y human neuroblastoma cells. The ED50 for this effect is 0.750-9.00 ng/mL in the presence of Recombinant Human GFR alpha -1/GDNF R alpha 1 Fc Chimera (Catalog # 714-GR ). The specific activity of recombinant human GDNF is >5.0 x 105 units/mg, which is calibrated against the human GDNF Reference Standard (NIBSC code: 09/266).

Specifications

• Accession Number: NP_000505.1
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Gene ID (Entrez): 2668
• Molecular Weight (g/mol): MolecularWeight-observed: 8 kDa, under reducing conditions., MolecularWeight-theroretical: 11.3 kDa
• Quantity: 50 μg
• Source: E. coli-derived human GDNF protein Gly113 - Ile211 with & without an N-terminal Met Produced using non-animal reagents in an animal-free laboratory. Manufactured and tested under cGMP guidelines.
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. A minimum of 12 months when stored at ≤ -20 °C as supplied. Refer to lot specific COA for the Use by Date. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, ≤ -20 °C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU per 1 μg of the protein by the LAL method.
• Gene Alias: Astrocyte-derived trophic factor, ATF, ATF1, ATF2, glial cell derived neurotrophic factor, glial cell line derived neurotrophic factor, glial cell line-derived neurotrophic factor, HFB1-GDNF, HGDNF, HSCR3
• Conjugate: Unconjugated
• Reconstitution: Reconstitute at 500 μg/mL in sterile water.
• Purity or Quality Grade: >97%, by SDS-PAGE with quantitative densitometry by Coomassie™ Blue Staining. The molecular weight by mass spectrometry is 22225 Da ± 5 Da, N-terminal Met species are also present (22356 ± 5 Da)
• Protein: GDNF