R&D Systems™ Recombinant Human GDNF Protein

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity, Binding Activity

Measured in a cell proliferation assay using SH-SY5Y human neuroblastoma cells. The ED50 for this effect is 2-12 ng/mL in the presence of Recombinant Human GFR alpha-1/GDNF R alpha-1 Fc Chimera (Catalog # 714-GR ). Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human GFR alpha-1/GDNF R alpha-1 Fc Chimera (Catalog # 714-GR ) at 1 μg/mL can bind Recombinant Human GDNF with an apparent Kd <1 nM.

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Specifications

• Formulation: Lyophilized from a 0.2μm filtered solution in PBS with BSA as a carrier protein.
• Gene ID (Entrez): 2668
• Molecular Weight (g/mol): 11.6 kDa (monomer)
• Name: GDNF
• Quantity: 10 μg
• Source: Mouse myeloma cell line,NS0-derived human GDNF protein Arg109-Ile211
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70° C as supplied. 1 month, 2 to 8° C under sterile conditions after reconstitution. 3 months, -20 to -70° C under sterile conditions after reconstitution.
• Structural Form: Disulfide-linked homodimer
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Purity or Quality Grade: 97%, by SDS-PAGE under reducing conditions and visualized by silver stain.