R&D Systems™ Recombinant Human GST-MDM2/HDM2 Protein

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity

Recombinant Human GST-Mdm2 is a Ubiquitin ligase (E3) that functions downstream of a Ubiquitin-activating (E1) enzyme and a Ubiquitin-conjugating (E2) enzyme to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human GST-Mdm2 concentration of 0.5-4 μM.

Specifications

• Accession Number: Q00987-1
• For Use With (Application): Bioactivity
• Formulation: X mg/mL (X μM) in 50 mM Hepes pH 8, 200 mM NaCl, 10% glycerol, 5 mM TCEP
• Gene ID (Entrez): 4193
• Molecular Weight (g/mol): M.W. (theoretical): 82 kDa
• Name: GST MDM2/HDM2
• Quantity: 50 μg
• Source: E. coli-derived human MDM2/HDM2 protein Contains an N-terminal GST (glutathione S-transferase) tag
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -70°C as supplied. 3 months, -70°C under sterile conditions after opening.
• Gene Symbol: MDM2
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Purity or Quality Grade: >85%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie™ Blue stain.
• Protein: MDM2/HDM2