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R&D Systems™ Recombinant Human Guanylyl Cyclase C His Avi-tag Protein

Catalog No. AVI2157050
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50 μg
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AVI2157050 50 μg
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Description

Biotinylated/GUCY2C His-tag

Measured by its binding ability in a functional ELISA. When Human Guanylyl Cyclase C Antibody (Catalog # MAB2157 ) is immobilized at 100 ng/mL (100 μL/well), Biotinylated Recombinant Human Guanylyl Cyclase C His-tag Avi-tag binds with an ED50 of 0.250-1.50 ng/mL. Measured by its ability to inhibit neurite outgrowth of E16-E18 rat embryonic cortical neurons. 2.50 μg/mL of protein is able to significantly inhibit neurite outgrowth.

Specifications

Accession Number P25092.2
For Use With (Application) Bioactivity
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Gene ID (Entrez) 2984
Molecular Weight (g/mol) MolecularWeight-observed: 75-85 kDa, under reducing conditions.; MolecularWeight-theroretical: 49 kDa
Quantity 50 μg
Source Human embryonic kidney cell, HEK293-derived human Guanylyl Cyclase C/GUCY2C protein Human Guanylyl Cyclase C Protein (Ser24-Gln430) (N-terminus) Accession # P25092.2 6-His tag Avi-tag (C-terminus)
Storage Requirements Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
Endotoxin Concentration <0.10 EU / 1 μg of the protein by the LAL method.
Gene Alias DIAR6, EC 4.6.1, GCC, GC-C, guanylate cyclase 2 C (heat stable enterotoxin receptor), Guanylate Cyclase C, Guanylyl cyclase C, GUC2C, GUC2CEC 4.6.1.2, GUCY2C, heat-stable enterotoxin receptor, hSTAR, Intestinal guanylate cyclase, MUCIL, STA Receptor, StAR, STARSTA receptor
Conjugate Biotin
Reconstitution Reconstitute at 1.00 mg/mL in PBS.
Purity or Quality Grade >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
Protein GUCY2C
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