R&D Systems™ Recombinant Human Heparan Sulfate-6-O-Sulfotransferase-3

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility.

Measured by its ability to transfer sulfate from PAPS to heparan sulfate. The specific activity is >65 pmol/min/μg, as measured under the described conditions.

Specifications

• Accession Number: Q8IZP7
• For Use With (Application): Enzyme Activity
• Formulation: Supplied as a 0.2 μm filtered solution in Tris and NaCl.
• Gene ID (Entrez): 266722
• Molecular Weight (g/mol): M.W. (Observed): 60-95 kDa, reducing conditions; M.W. (theoretical): 52 kDa
• Quantity: 20 μg
• Source: Chinese Hamster Ovary cell line, CHO-derived human Heparan Sulfate 6-O-Sulfotransferase 3/HS6ST3 protein Pro28-Trp471, with an N-terminal 6-His tag
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20°C to -70°C as supplied. 3 months, -20°C to -70°C under sterile conditions after opening.
• Endotoxin Concentration: <1.0 EU / 1 μg of the protein by the LAL method.
• Gene Alias: DKFZp761K2315, heparan sulfate 6-O-sulfotransferase 3
• Common Name: Heparan Sulfate 6-O-Sulfotransferase 3/HS6ST3
• Gene Symbol: HS6ST3
• Biological Activity: Measured by its ability to transfer sulfate from PAPS to heparan sulfate. The specific activity is >65 pmol/min/μg, as measured under the described conditions.
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Expression System: Chinese Hamster Ovary cell line, CHO-derived Pro28-Trp471, with an N-terminal 6-His tag
• Purity or Quality Grade: >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie™ Blue stain (at 5 μg / lane.)
• Protein: Heparan Sulfate 6-O-Sulfotransferase 3/HS6ST3