R&D Systems™ Recombinant Human HGF (NS0-expressed) Biotin Protein

Description

Biotinylated

Measured by its binding ability in a functional ELISA. When Biotinylated Recombinant Human HGF Prorein (Catalog # BT294) is captured on EvenCoat Streptavidin Coated Plates (Catalog # CP004 ) at 0.500 μg/mL (100 μL/well), Recombinant Human HGFR/c-MET Fc Chimera Protein (Catalog # 8614-MT ) binds with an ED50 of 6.00-60.0 ng/mL.

Specifications

• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Gene ID (Entrez): 3082
• Molecular Weight (g/mol): M.W.-observed: 55-69 kDa and 24-39 kDa, under reducing conditions., M.W.-theroretical: alpha chain: 53.7 kDa beta chain: 26 kDa
• Quantity: 25 μg
• Source: Mouse myeloma cell line, NS0-derived human HGF protein Gln32-Ser728 (Asp384Asn, Asp416Asn, Leu622Ser, His645Arg, Val678Ile)
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Gene Alias: deafness, autosomal recessive 39, DFNB39, EC 3.4.21, EC 3.4.21.7, fibroblast-derived tumor cytotoxic factor, F-TCF, hepatocyte growth factor (hepapoietin A; scatter factor), Hepatopoeitin-A, HGFB, HPTAhepatocyte growth factor, lung fibroblast-derived mitogen, Scatter factor, SFhepatopoeitin-A
• Conjugate: Biotin
• Reconstitution: Reconstitute at 250 μg/mL in PBS.
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: HGF