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R&D Systems™ Recombinant Human HGF (NS0-expressed) Protein

Human Recombinant Protein

Manufacturer:  R&D Systems™ 294HGN250

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Catalog No. 29-4HGN-250

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Measured by its ability to induce IL-11 secretion by Saos‑2 human osteosarcoma cells. The ED50 for this effect is 0.1-0.5 ng/mL.
The specific activity of recombinant human HGF is approximately 1.5 x 103 IU/µg, which is calibrated against recombinant human HGF WHO International Standard (NIBSC code: 96/564).

Source:  Mouse myeloma cell line, NS0-derived human HGF protein



Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
alpha chain: 53.7 kDa beta chain: 26 kDa
250 μg
Manual defrost freezer; Avoid repeated freeze-thaw cycles; 12 mo. from receipt, -20 to -70°C as supplied; 1 mo., 2 to 8°C under sterile conditions after reconstitution; 3 mo., -20 to -70°C under sterile conditions after reconstitution
<0.10 EU per 1 μg of the protein by the LAL method.
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Mouse myeloma cell line, NS0-derived human HGF protein Gln32-Ser728
Reconstitute at 50 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
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