R&D Systems™ Recombinant Human HGF Protein, CF

Description

Measured by its ability to induce IL-11 secretion by Saos-2 human osteosarcoma cells. The specific activity of Recombinant Human HGF is approximately 1.5 x 103 IU/μg, which is calibrated against recombinant human HGF WHO International Standard (NIBSC code: 96/564).

• The ED50 for this effect is 0.1 to 0.5 ng/mL.
• The specific activity of Recombinant Human HGF is approximately 1.5 x 103 IU/μg, which is calibrated against recombinant human HGF WHO International Standard (NIBSC code: 96/564).

Specifications

• Accession Number: P14210.2
• For Use With (Application): Bioactivity
• Gene ID (Entrez): 3082
• Molecular Weight (g/mol): 53.7 kDa ( alpha chain), 26 kDa ( beta chain)
• Name: deafness, autosomal recessive 39, DFNB39, EC 3.4.21, EC 3.4.21.7, fibroblast-derived tumor cytotoxic factor, F-TCF, hepatocyte growth factor (hepapoietin A; scatter factor), Hepatopoeitin-A, Hepatopoietin A, HGFB, HPTA, HPTAhepatocyte growth factor, lung fibroblast-derived mitogen, Scatter factor, SF, SFhepatopoeitin-A
• Quantity: 100 μg
• Source: Spodoptera frugiperda, Sf 21 (baculovirus)-derived human HGF protein Gln32-Ser728 (Asp384Asn, Asp416Asn, Leu622Ser, His645Arg, Val678Ile)
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Gene Symbol: HGF
• Conjugate: Unconjugated
• Species: Human
• Reconstitution: Reconstitute at 100 μg/mL in sterile PBS.
• Buffer: Lyophilized from a 0.2 μm filtered solution in NaH2PO4 and NaCl.
• Endotoxin Level: <1.0 EU per 1 μg of the protein by the LAL method.
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.