R&D Systems™ Recombinant Human His6-NEDD4 Protein

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility.

Recombinant Human NEDD 4 is a Ubiquitin Ligase (E3) that functions downstream of a Ubiquitin-activating (E1) enzyme and a Ubiquitin-conjugating (E2) enzyme to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application.

Specifications

• Accession Number: P46934
• For Use With (Application): Bioactivity
• Formulation: X mg/mL (X μM) in 50 mM HEPES pH 8.0, 100 mM NaCl, 5 mM DTT, 1 mM EDTA
• Gene ID (Entrez): 4734
• Molecular Weight (g/mol): M.W. (theoretical): 88 kDa
• Name: Cell proliferation-inducing gene 53 protein, E3 ubiquitin-protein ligase NEDD4, EC 6.3.2, EC 6.3.2.-, KIAA0093NEDD4-1MGC176705, NEDD-4, Neural precursor cell expressed developmentally down-regulated protein 4, neural precursor cell expressed, developmentally down-regulated 4, receptor-potentiating factor 1, RPF1
• Quantity: 50 μg
• Source: E. coli-derived human NEDD4 protein Contains a N-terminal 6-His tag, Leu570 - Asp1319
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -70°C as supplied. 3 months, -70°C under sterile conditions after opening.
• Conjugate: Unconjugated
• Species: Human
• Purity or Quality Grade: >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie™ Blue stain.
• Protein: NEDD4