R&D Systems™ Recombinant Human His6-Ubiquitin E1 Enzyme (UBE1)

Catalog No.	Quantity
E304050	50 μg

Catalog No. E304050 Supplier R&D Systems™ Supplier No. E304050/DISC

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Enzyme Activity

Recombinant Human His6-Ubiquitin Activating Enzyme (UBE1) is a member of the Ubiquitin-activating (E1) enzyme family that is required for the first step of the enzymatic cascade that subsequently utilizes a Ubiquitin-conjugating (E2) enzyme and a Ubiquitin ligase (E3) to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human His6-Ubiquitin Activating Enzyme (UBE1) concentration of 50-200 nM.

Specifications

• Accession Number: P22314.3
• For Use With (Application): Enzyme Activity
• Formulation: Supplied as a 0.2 μm filtered solution in HEPES, NaCl and TCEP.
• Gene ID (Entrez): 7317
• Molecular Weight (g/mol): M.W. (theoretical): 121 kDa
• Name: His6 Ubiquitin-activating Enzyme/UBE1
• Quantity: 50 μg
• Source: Spodoptera frugiperda, Sf 21 (baculovirus)-derived human Ubiquitin-activating Enzyme/UBE1 protein Contains an N-terminal Met-Ser-Tyr-Tyr and 6-His tag
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -70°C as supplied. 3 months, -70°C under sterile conditions after opening.
• Gene Symbol: UBA1
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Purity or Quality Grade: >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie™ Blue stain.
• Protein: Ubiquitin-activating Enzyme/UBE1