R&D Systems™ Recombinant Human His6-USP30 Protein

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility.

Reaction conditions will need to be optimized for each specific application. We recommend an initial USP30 concentration of 20-100 nM in reactions utilizing Ubiquitin-AMC or Ubiquitin-Rhodamine substrates (U-550 or U-555), or 200-500 nM when digesting recombinant Polyubiquitin chain substrates.

Specifications

• Accession Number: Q70CQ3.1
• For Use With (Application): Bioactivity
• Formulation: Supplied as a 0.2 μm filtered solution in HEPES, NaCl, Glycerol and DTT.
• Gene ID (Entrez): 84749
• Molecular Weight (g/mol): M.W. (theoretical): 54 kDa
• Quantity: 50 μg
• Source: Spodoptera frugiperda, Sf 21 (baculovirus)-derived human USP30 protein Thr57 - Glu517 with a N-terminal Met and a C-terminal 6-His tag
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -70°C as supplied. 3 months, -70°C under sterile conditions after opening.
• Gene Alias: Deubiquitinating enzyme 30, EC 3.1.2.15, EC 3.4.19.12, FLJ40511, MGC10702, ubiquitin carboxyl-terminal hydrolase 30, ubiquitin specific peptidase 30, ubiquitin specific protease 30, ubiquitin thioesterase 30, Ubiquitin thiolesterase 30, ubiquitin-specific protease 30, Ubiquitin-specific-processing protease 30, Ub-specific protease 30
• Common Name: USP30
• Gene Symbol: USP30
• Biological Activity: Reaction conditions will need to be optimized for each specific application. We recommend an initial USP30 concentration of 20-100 nM in reactions utilizing Ubiquitin-AMC or Ubiquitin-Rhodamine substrates (U-550 or U-555), or 200-500 nM when digesting recombinant Polyubiquitin chain substrates.
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Expression System: Spodoptera frugiperda, Sf 21 (baculovirus)-derived Contains a C-terminal 6-His tag, Thr57 - Glu517 Accession # Q70CQ3
• Purity or Quality Grade: >98%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie™ Blue stain.
• Protein: USP30