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R&D Systems™ Recombinant Human His6-USP4 Protein

Catalog No. E596050
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E596050 50μg
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Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility.

Reaction conditions will need to be optimized for each specific application. We recommend an initial recombinant human USP4 concentration of 10-100 nM when using Ubiquitin-AMC or Ubiquitin-Rh110 substrates (U-550, U-555)

Specifications

Accession Number Q13107
For Use With (Application) Bioactivity
Formulation X mg/mL (X μM) in 50 mM HEPES pH 7.5, 100 mM NaCl, 1 mM TCEP, 10% (v/v) Glycerol
Gene ID (Entrez) 7375
Molecular Weight (g/mol) M.W. (theoretical): 109 kDa
Quantity 50 μg
Source Spodoptera frugiperda, Sf 21 (baculovirus)-derived human USP4 protein Contains an C-terminal 6-His tag
Storage Requirements Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -70°C as supplied. 3 months, -70°C under sterile conditions after opening.
Gene Alias Deubiquitinating enzyme 4, EC 3.1.2.15, EC 3.4.19.12, MGC149848, MGC149849, ubiquitin carboxyl-terminal esterase 4, ubiquitin carboxyl-terminal hydrolase 4, ubiquitin specific peptidase 4 (proto-oncogene), ubiquitin specific protease 4 (proto-oncogene), ubiquitin thioesterase 4, Ubiquitin thiolesterase 4, ubiquitin-specific processing protease 4, Ubiquitin-specific-processing protease 4, Ubiquitous nuclear protein homolog, UNP, Unph, UNPUnph
Common Name USP4
Gene Symbol USP4
Biological Activity Reaction conditions will need to be optimized for each specific application. We recommend an initial recombinant human USP4 concentration of 10-100 nM when using Ubiquitin-AMC or Ubiquitin-Rh110 substrates (U-550, U-555)
Conjugate Unconjugated
Species Human
Recombinant Recombinant
Expression System Spodoptera frugiperda, Sf 21 (baculovirus)-derived Contains an C-terminal 6-His tag
Purity or Quality Grade >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie™ Blue stain.
Protein USP4
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