R&D Systems™ Recombinant Human HMGB1 Protein

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity

Measured by its ability to induce TNF-alpha secretion by RAW 264.7 mouse monocyte/macrophage cells. The ED50 for this effect is 2.5-12.5 μg/mL.

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Specifications

• Accession Number: P09429
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS.
• Gene ID (Entrez): 3146
• Molecular Weight (g/mol): M.W. (Observed): 28-34 kDa, reducing conditions; M.W. (theoretical): 25 kDa
• Name: HMGB1/HMG-1
• Preparation Method: Reconstitute at 200μg/mL in PBS.
• Quality Control Testing: SDS-PAGE: 28-34kDa, reducing conditions
• Quantity: 50 μg
• Source: Mouse myeloma cell line, NS0-derived human HMGB1/HMG-1 protein Met1-Glu215
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Regulatory Status: RUO
• Termination: Met1 & Gly2
• Without Additives: Carrier Protein
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Gene Symbol: HMGB1
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Reconstitution: Reconstitute at 200 μg/mL in PBS.
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: HMGB1/HMG-1